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rabbit anti rfp polyclonal  (OriGene)


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    Structured Review

    OriGene rabbit anti rfp polyclonal
    Rabbit Anti Rfp Polyclonal, supplied by OriGene, used in various techniques. Bioz Stars score: 94/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+serum/RFP-Tag+(Ads%2E+to+Hu%2C+Ms%2C+Rt+Serum+Proteins)+Rabbit+Polyclonal+Antibody/pmc13196551-5-0-4
    Average 94 stars, based on 6 article reviews
    rabbit anti rfp polyclonal - by Bioz Stars, 2026-10
    94/100 stars

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    Incubation:

    Article Title: Artemisiae Scopariae Herba (Yinchen) suppresses ferroptosis in mice with osteoporosis via the Nrf2/Slc7a11/Gpx4 pathway
    Article Snippet: The sections were treated with color developing solution (DAB Kit; cat. no. ZLI-9017; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.). .. Subsequently, the sections were blocked with 10% rabbit serum (cat. no. ZLI-9026; Beijing Zhongshan Jinqiao Biotechnology Co., Ltd.) at room temperature for 30 min, and incubated with Gpx4 monoclonal antibody (1:50; cat. no. 67763-1-Ig; Proteintech Group, Inc.) and OCN antibody (1:100; cat. no. ab93876; Abcam) at 4°C overnight. .. The corresponding fluorescent secondary antibodies (Alexa Fluor 488-conjugated goat anti-mouse IgG, 1:200, cat. no. ab150113, Abcam; Alexa Fluor 594-conjugated goat anti-rabbit IgG, 1:200, cat. no. ab150080, both Abcam) were added at room temperature for 50 min in the dark, followed by tyramide signal amplification reaction at room temperature for 10 min in the dark.

    Article Title: BMP7 regulates lung fibroblast proliferation in newborn rats with bronchopulmonary dysplasia.
    Article Snippet: The sections were dewaxed, incubated in 3% H2O2 for 15 min to eliminate endogenous peroxidase activity, and incubated in pancreatin (Gibco; Thermo Fisher Scientific, Inc., Waltham, MA, USA) for 15 min at room temperature. .. The sections were washed three times with PBS, blocked with rabbit serum (OriGene Technologies, Inc., Beijing, China) and incubated with a primary antibody targeting BMP7 (cat. no. ab56023; 1:125; Abcam, Cambridge, UK) overnight at 4 ̊C. .. The tissues were washed 3 times with PBS, and processed following the protocols provided in the UltraSensitive serum amyloid P (goat) immunohistochemistry kit (OriGene Technologies, Inc., Beijing, China).

    Article Title: Overexpression of Epsin 3 enhances migration and invasion of glioma cells by inducing epithelial‑mesenchymal transition.
    Article Snippet: .. Endogenous peroxidase activity was blocked by incubating the slides in 3% hydrogen peroxide for 15 min. following heat-induced antigen retrieval at 100 ̊C for 10 min, sections were incubated with 5% rabbit serum (ZSGB-BIO; OriGene Technologies, Inc.) for 10 min to eliminate non‐specific binding. ..

    Article Title: <p>Artemisiae Scopariae Herba (Yinchen) suppresses ferroptosis in mice with osteoporosis via the Nrf2/Slc7a11/Gpx4 pathway</p>.
    Article Snippet: .. Subsequently, the sections were blocked with 10% rabbit serum (cat. no. Zli‐9026; Beijing Zhongshan Jinqiao Biotechnology co., ltd.) at room temperature for 30 min, and incubated with Gpx4 monoclonal antibody (1:50; cat. no. 67763‐1‐ig; Proteintech Group, inc.) and OCN antibody (1:100; cat. no. ab93876; Abcam) at 4 ̊C overnight. .. The corresponding fluorescent secondary anti‐ bodies (alexa Fluor 488‐conjugated goat anti‐mouse igG, 1:200, cat. no. ab150113, abcam; alexa Fluor 594‐conjugated goat anti‐rabbit igG, 1:200, cat. no. ab150080, both abcam) were added at room temperature for 50 min in the dark, followed by tyramide signal amplification reaction at room temperature for 10 min in the dark.

    Article Title: Further Evaluation of the Mother-to-Child Transmission Potential of SARS-CoV-2 Infection During Pregnancy: A Retrospective Study
    Article Snippet: .. Sections were blocked with rabbit serum and incubated with antibodies against ACE2 (OriGene Technologies, Rockville, Maryland, USA). ..

    Article Title: The pathophysiological nature of sarcomeres in trigger points in patients with myofascial pain syndrome: A preliminary study
    Article Snippet: After being rinsed twice in phosphate‐buffered saline (PBS), the slices were placed in a wet chamber and incubated in 3% hydrogen peroxide for 10 min. After being washed with PBS three times, the sections were incubated with 10% normal goat serum at 37°C for 30 min, then diluted with the following antibodies at 4°C overnight: anti‐Eph receptor B1 + Eph receptor B2 (phospho Y594 + Y604) antibody (Abcam ab61791 1:300), anti‐RhoA (Abcam EP487Y 1:200), anti‐Rac1 (Abcam ab64533 1:150) and anti‐Cdc42 (cell division control protein 42; Abcam ab180785 1:300). .. After being washed with PBS three times, the slices were incubated with biotin‐labelled goat anti‐rabbit serum at 37°C for 30 min, then treated with a horseradish peroxidase‐labelled streptavidin complex at 37°C for 30 min (Origene PV9000). ..

    Activity Assay:

    Article Title: Overexpression of Epsin 3 enhances migration and invasion of glioma cells by inducing epithelial‑mesenchymal transition.
    Article Snippet: .. Endogenous peroxidase activity was blocked by incubating the slides in 3% hydrogen peroxide for 15 min. following heat-induced antigen retrieval at 100 ̊C for 10 min, sections were incubated with 5% rabbit serum (ZSGB-BIO; OriGene Technologies, Inc.) for 10 min to eliminate non‐specific binding. ..

    Binding Assay:

    Article Title: Overexpression of Epsin 3 enhances migration and invasion of glioma cells by inducing epithelial‑mesenchymal transition.
    Article Snippet: .. Endogenous peroxidase activity was blocked by incubating the slides in 3% hydrogen peroxide for 15 min. following heat-induced antigen retrieval at 100 ̊C for 10 min, sections were incubated with 5% rabbit serum (ZSGB-BIO; OriGene Technologies, Inc.) for 10 min to eliminate non‐specific binding. ..



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